An Unbiased Approach of Sampling TEM Sections in Neuroscience

7.1K Views

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10:56 min

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April 13th, 2019

DOI :

10.3791/58745-v

April 13th, 2019

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Stefan Wernitznig¹, Florian Reichmann², Mariella Sele¹, Christoph Birkl³, Johannes Haybäck⁴^,⁵, Florian Kleinegger⁴, Anna Birkl-Töglhofer⁴, Stefanie Krassnig⁴, Christina Wodlej⁴, Peter Holzer², Daniel Kummer¹, Elisabeth Bock¹, Gerd Leitinger¹

¹Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, ²Department of Pharmacology, Otto Loewi Research Center, Medical University of Graz, ³Division of General Neurology, Department of Neurology, Medical University of Graz, ⁴Institute of Pathology, Medical University of Graz, ⁵Department of Pathology, Medical Faculty, Otto von Guericke University Magdeburg

Summary

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Unbiased Sampling Protocol

Transmission Electron Microscope

TEM Serial Section Software

ROI region Of Interest

Random Point Sampling Software

Chapters in this video

0:04

Title

1:02

TEM Preparation Embedding, Ultra-Thin Sectioning and Staining

2:05

Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages

5:25

Analyze the TEM images with ImageJ to Document Ultrastructural Features

7:10

Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph

9:19

Results: Unbiased Approach of Sampling TEM Sections

10:25

Conclusion

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