Sign In
JoVE Journal
Biochemistry
You have full access to this content through
Nanyang Technological UniversityCapture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
9.3K Views
•
09:36 min
•
October 19th, 2018
DOI :
October 19th, 2018
•1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
Chapters in this video
0:04
Title
0:38
Preparation of Lysate of Metabolically Labeled and UV Cross-linked Cells
3:33
Preparation of Samples for RNA-interactome Capture
5:13
RNA-interactome Capture
7:45
Results: Quality Control of Captured RNA-binding Proteins
8:53
Conclusion
Related Videos
10:37
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
14.9K Views
10:49
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
12.5K Views
09:43
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
8.6K Views
10:52
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
8.0K Views
12:29
mRNA Interactome Capture from Plant Protoplasts
9.0K Views
07:31
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
5.6K Views
08:07
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
8.4K Views
07:53
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
9.1K Views
08:31
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
9.8K Views
05:22
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
3.5K Views
ISSN 2578-2037
Copyright © 2025 MyJoVE Corporation. All rights reserved
We use cookies to enhance your experience on our website.
By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.