Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
8.2K Views
•
08:40 min
•
February 14th, 2019
DOI :
February 14th, 2019
•Chapters in this video
0:04
Title
0:50
Isolation of Crude Thylakoid Membranes from Spinach Leaves
2:51
Solubilization of Thylakoid Membranes for Loading onto Native Gels and Separation of Solubilized Thylakoid Proteins by Native Gel Electrophoresis
4:02
Excision of Thylakoid Complex Bands from Native Green Gels and Collection of Room Temperature Steady-State Fluorescence Spectra
5:01
Time-correlated Single Photon Counting of Green Gel Bands and Data Analysis
6:07
Results: Native Green Gel Electrophoresis and Band Characterization
8:14
Conclusion
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