Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

11.2K Views

•

07:49 min

•

August 3rd, 2018

DOI :

10.3791/57577-v

August 3rd, 2018

•

Hiroshi I. Suzuki¹, Masafumi Horie²^,³, Hajime Mihira⁴, Akira Saito²

¹David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, ²Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, ³Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, ⁴Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo

Summary

Explore More Videos

Keywords Endothelial mesenchymal Transition

Endothelial Plasticity

In Vitro EndMT

MS 1 Endothelial Cells

Immunofluorescence Staining

Phallotoxin

Nuclear Staining

Chapters in this video

0:04

Title

0:41

Induction of EndMT

1:58

Immunocytochemical Analysis

5:02

Three-dimensional Collagen Gel Contraction Assay

6:09

Results: Use of an In Vitro Method to Study Endothelial-mesenchymal Transition Upon TGF-β Treatment

7:20

Conclusion

Related Videos

09:34

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

16.7K Views

13:11

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth

12.7K Views

10:28

Herbivore-induced Blueberry Volatiles and Intra-plant Signaling

16.3K Views

10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

10.3K Views

10:46

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

16.2K Views

10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

35.6K Views

11:44

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

14.0K Views

07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

8.7K Views

08:02

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

9.6K Views

09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

8.5K Views