JoVE Journal

Chemistry

This content is Open Access.

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Combined precursor isotopic labeling and isobaric tagging (cPILOT) is an enhanced sample multiplexing strategy that is capable of increasing the number of samples that can be analyzed simultaneously with available isobaric tags. Incorporation of a robotic platform has greatly increased experimental throughput, reproducibility, and quantitative accuracy.

Chapters in this video

0:04

Introduction

0:51

Protein Extraction and Sample Preparation

3:48

Desalting Step 1

4:39

Dimethylation Labeling and Isobaric Tagging

7:21

Results: Automated cPILOT Processing of 22 Samples

8:47

Conclusion

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