Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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11.8K Views

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10:37 min

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November 15th, 2017

DOI :

10.3791/56474-v

November 15th, 2017

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Anthony A. High¹, Haiyan Tan¹, Vishwajeeth R. Pagala¹, Mingming Niu²^,³, Ji-Hoon Cho¹, Xusheng Wang¹, Bing Bai², Junmin Peng¹^,²

¹St. Jude Proteomics Facility, St. Jude Children's Research Hospital, ²Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, ³Heilongjiang University of Chinese Medicine

Podsumowanie

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Keywords Proteome Profiling

Isobaric Labeling

Liquid Chromatography

Mass Spectrometry

Quantification

Protein Identification

Protein Quantitation

Biomarker Discovery

Cell Lysis

Tissue Homogenization

Rozdziały w tym wideo

0:05

Title

1:00

Preparation of Cells/Tissues

1:45

Protein Extraction, Quality Control Western Blotting, In-solution Digestion, and Peptide Desalting

4:45

TMT Labeling of Peptides

5:50

Extensive High-resolution, Basic pH LC Prefactionation

6:43

LC-MS/MS Preparation and Parameters

7:41

Results: Deep Proteome Profiling Analysis

8:45

Conclusion

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