JoVE Journal

Immunology and Infection

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Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

9.5K Views

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07:19 min

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October 18th, 2016

DOI :

10.3791/54677-v

October 18th, 2016

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Salome Murinello¹, Stacey K. Moreno¹, Matthew S. Macauley², Susumu Sakimoto¹, Peter D. Westenskow¹^,³, Martin Friedlander¹

¹Department of Cell and Molecular Biology, The Scripps Research Institute, ²Department of Chemical Physiology, The Scripps Research Institute, ³The Lowy Medical Research Institute

Summary

Microglial phagocytosis is critical for the maintenance of tissue homeostasis and inadequate phagocytic function has been implicated in pathology. However, assessing microglia function in vivo is technically challenging. We have developed a simple but robust technique for precisely monitoring and quantifying the phagocytic potential of microglia in a physiological setting.

Explore More Videos

Retinal Microglia

Phagocytic Function

Intravitreal Injection

Fluorescent Particles

Chapters in this video

0:05

Title

0:47

Intravitreal Bead Injection

2:48

Retinal Tissue Harvest

4:19

Single Cell Suspension Staining for Flow Cytometric Analysis

5:49

Results: Representative Microglia Phagocytic Function after LPS Challenge in Pup and Adult Mice

6:30

Conclusion

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