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Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

9.4K Views

10:58 min

March 29th, 2017

DOI :

10.3791/54667-v

March 29th, 2017


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Keywords Lipid Alteration

Chapters in this video

0:05

Title

1:14

Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation

3:59

Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils

5:33

High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids

7:53

High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol

8:48

Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis

9:33

Conclusion

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