JoVE Journal

Immunology and Infection

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Nanyang Technological University

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Here, we present a protocol for generating neutrophil extracellular traps (NETs) and operating NETQUANT, a fully automatic software option for quantification of NETs in immunofluorescence images.

Chapters in this video

0:04

Title

0:34

Analysis and Quantification of Neutrophil Extracellular Traps (NETs)

2:19

Segmentation of Cells in the DNA Channel and the NET Channel

3:41

Single-cell Analysis of Identifiable Properties

4:19

Comparison of Cell Properties to Identify NET-forming Cells

5:23

Result Outputs Demeanor

6:16

Results: Analysis of NET Formation

7:07

Conclusion

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