Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
8.4K Views
•
10:00 min
•
November 8th, 2016
DOI :
November 8th, 2016
•Chapters in this video
0:05
Title
1:00
Isolation and Activation of Human Naïve CD4+ T Cells
4:22
Collection and Fixation of Resting and Activated Naïve CD4+ T Cells
5:34
Staining, Mounting and Visualization by Confocal Microscopy
7:47
Results: Localization of GD3 and GD2 Gangliosides in Adhered Activated CD4+ T Cells
9:13
Conclusion
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