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Nanyang Technological UniversityFunctional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
17.6K Views
•
09:27 min
•
June 24th, 2016
DOI :
June 24th, 2016
•Chapters in this video
0:05
Title
1:13
Preparation of Electro-competent Bacterial Cells for Transformation
2:33
Electroporation of Bacterial Cells with Complementation Plasmids
3:39
Transformation of AOH1 to Facilitate Functional Complementation Using L,L-diaminopimelate Aminotransferase
4:18
Transformation of TKL-11 to Facilitate Functional Complementation Using UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate Ligase
5:12
Transformation of Mutant Hx699 to Facilitate Functional Complementation of Hypomucoid Phenotype of Novosphingobium sp. Strain Hx699
6:02
Results: Functional Complementation Analysis
7:44
Conclusion
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