Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

13.0K Views

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14:13 min

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January 1st, 2018

DOI :

10.3791/55754-v

January 1st, 2018

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Thea M. Edwards¹^,², Howard E. Morgan²^,³, Coralia Balasca⁴, Naveen K. Chalasani⁵, Lauren Yam⁴^,⁶, Alison M. Roark⁴

¹Department of Biology, University of the South, ²School of Biological Sciences, Louisiana Tech University, ³School of Medicine, Louisiana State University Health Sciences Center, ⁴Department of Biology, Furman University, ⁵Department of Computer Science, Louisiana Tech University, ⁶Clemson University

Summary

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Keywords Yeast Estrogen Screen

Personal Care Products

Environmental Estrogens

Endocrine Disruption

Ecophysiology

In Vitro

Undergraduate Teaching

Chapters in this video

0:05

Title

1:02

Preparing Reagents, Samples, and Extraction Controls

3:23

Subculturing Yeast and Preparing YES Plates

7:18

Processing YES Plates (Day 2 of the Assay)

9:26

Calculating LacZ Values

10:28

Interpolating Sample Estradiol Equivalents (EEQs) using 4-parameter Logistic Regression

11:30

Results: Yeast Estrogen Screen Quantifies Ligands in Personal Care Products (PCPs)

12:52

Conclusion

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