En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

9.5K Views

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08:58 min

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February 25th, 2016

DOI :

10.3791/53718-v

February 25th, 2016

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Yi Yu¹^,², Yuyan Xiong¹^,², Jean-Pierre Montani²^,³, Zhihong Yang¹^,², Xiu-Fen Ming¹^,²

¹Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, ²Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, ³System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg

Summary

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Keywords Nitric Oxide

Cardiovascular Physiology

Endothelial Dysfunction

ENOS Uncoupling

Organ Bath System

Krebs Ringer Bicarbonate Buffer

Aortic Rings

Dissection

Fluorescent Staining

Chapters in this video

0:05

Title

0:52

Preparing the Organ Bath System

2:01

Isolation of Mouse Aortas

3:03

DHE and DAF-2DA Staining

4:47

En Face Mounting and Confocal Microscopic Imaging

6:42

Results: eNOS-uncoupling in Obese Mice

7:56

Conclusion

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