The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

33.2K Views

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05:44 min

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April 25th, 2016

DOI :

10.3791/53613-v

April 25th, 2016

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Anne-Sophie Perrin-Terrin¹^,², Florine Jeton¹^,³, Aurelien Pichon¹^,³^,⁴, Alain Frugière², Jean-Paul Richalet¹^,³, Laurence Bodineau², Nicolas Voituron¹^,³

¹Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363, University Paris 13, ²UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique, Sorbonne Universités, ³Laboratory of Excellence GR-Ex, ⁴Laboratory MOVE (EA 6314), University of Poitiers

Summary

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Keywords C FOS Protein

Immunohistochemical Detection

Neuronal Activity

Brain Mapping

Physiological Responses

In Vivo

Ex Vivo

Brain Tissue

Coronal Brain Stem Sections

Peroxidase Activity

Nonspecific Binding

Polyclonal Antibody

Biotinylated Antibody

Avidin Biotin Peroxidase Complex

DAB

Nickel Ammonium Sulfate

Hydrogen Peroxide

Chapters in this video

0:05

Title

0:44

Day 1 Endogenous Peroxidase Activity Destruction, Non-specific Binding Site Blockade and Incubation with the Primary Antibody

1:44

Day 3 Incubation with Secondary Antibody and Development of a Color Reaction

3:21

Sample Mounting

4:16

Results: c-FOS-positive Neurons on Sections of the Medulla Oblongata in Control Mice and Mice Submitted to Hypoxia In Vivo

5:17

Conclusion

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