Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

9.7K Views

•

13:26 min

•

March 31st, 2018

DOI :

10.3791/57153-v

March 31st, 2018

•

Jennifer Eom¹, Vaughan Feisst¹, Louis Ranjard², Kerry Loomes¹, Tanvi Damani¹, Victoria Jackson-Patel¹^,³, Michelle Locke⁴^,⁵, Hilary Sheppard¹, Pritika Narayan¹^,⁶, P. Rod Dunbar¹^,⁷

¹School of Biological Sciences, The University of Auckland, ²Research School of Biology, The Australian National University, ³Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, ⁴Department of Plastic Surgery, Counties Manukau District Health Board, ⁵Department of Surgery, Faculty of Medical and Health Sciences, The University of Auckland, ⁶Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences, The University of Auckland, ⁷Maurice Wilkins Centre, The University of Auckland

Summary

Explore More Videos

Keywords Mesenchymal Cell

Adipogenic Differentiation

Lineage specific Marker

Fatty Acid Binding Protein 4

Stromal Cells

High content Screening

Immunofluorescent Microscopy

Differentiation Medium

Chapters in this video

0:00

Title

1:55

Inducing the differentiation of human adipose derived stromal cells

3:36

Staining for differentiation

5:53

Imaging cells using high content screening microscope

8:23

Image Analysis: Cell Scoring

9:43

Image Analysis: Transfluor

10:43

Representative results

Related Videos

07:06

Principles of Site-Specific Recombinase (SSR) Technology

20.6K Views

12:03

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

14.5K Views

07:35

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

13.8K Views

11:37

Measurement of Cellular Chemotaxis with ECIS/Taxis

14.7K Views

10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

12.2K Views

05:56

Manipulating the Murine Lacrimal Gland

20.7K Views

11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

7.9K Views

08:00

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

11.4K Views

06:10

Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle

6.3K Views

06:46

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

8.3K Views