Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

2:17

Passivation of Glass Coverslips with PLL-PEG-biotin

4:04

Fabrication of Stamps for Microcontact Printing

4:38

Patterning Streptavidin or Adhesive Cues onto Passivated Glass Coverslips

6:13

Creating Adhesive Gradients onto Streptavidin-pattern Surfaces

7:47

Loading of Cells with a Soluble Gradient into the Microfluidics Device for Live Cell Imaging

9:08

Results: Cell Migration in Response to Adhesive and Soluble Gradients

12:16

Conclusion