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Bioengineering

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Nanyang Technological University

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

9.9K Views

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12:42 min

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December 22nd, 2015

DOI :

10.3791/53154-v

December 22nd, 2015

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Andrew Nickerson¹^,²^,³, Tao Huang¹^,²^,³, Li-Jung Lin¹^,²^,³, Xioalin Nan¹^,²^,³

¹Department of Biomedical Engineering, Oregon Health and Science University, ²Knight Cancer Institute, Oregon Health and Science University, ³OHSU Center for Spatial Systems Biomedicine, Oregon Health and Science University

Summary

Protein-protein interactions are visualized in cells with nanometer spatial resolution by combining bimolecular fluorescence complementation (BiFC) with photoactivated localization microscopy (PALM). Described here is the use of BiFC-PALM for imaging Ras-Raf interactions in U2OS cells for visualizing the nanoscale clustering and diffusion of individual Ras-Raf complexes.

Explore More Videos

Protein protein Interactions

Superresolution Microscopy

Photoactivated Localization Microscopy

Bimolecular Fluorescence Complementation

Single Molecule Imaging

Ras Raf Interaction

Chapters in this video

0:05

Title

0:40

Imaging Fixed Cells

3:57

Image Processing

7:46

Track Single Molecules in Live Cells

8:55

Results: Visualization of Individual Ras-Raf Interactions Using BiFC-PALM

11:02

Conclusion

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