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Nanyang Technological University

Fabrication and Use of MicroEnvironment microArrays (MEArrays)

9.9K Views

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11:57 min

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October 11th, 2012

DOI :

10.3791/4152-v

October 11th, 2012

•
1Life Science Division, Lawrence Berkeley National Laboratory, 2Department of Comparative Biochemistry, University of California, Berkeley

A combinatorial functional screening method for gaining insights into the impacts of the molecular composition of microenvironments on cellular functions is described. The method takes advantage of existing microarray-based technologies to generate arrays of defined combinatorial microenvironments that support cell adhesion and functional analysis.

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Fabrication
Use
MicroEnvironment MicroArrays
MEArrays
Interactions
Cells
Microenvironment
Functional Consequences
Single Cell Level
Differentiation
Migration
Proliferation Phenotypes
Tissue Level
Morphogenesis
Tumorigenesis
Cell cell Interactions
ECM Interactions
Soluble Factor Interactions
Physical Characteristics
Genomic Content
Combinatorial Interactions
Microenvironmental Components
Distinctive Behaviors
MEArray Platform
Cell based Functional Screening
Molecular Composition
Elastic Modulus
Microarray Technology
Micropatterning Technology

Chapters in this video

0:05

Title

1:28

Generating Substrate: Polydimethylsiloxane (PDMS) Slides

2:49

Generating Substrate: Polyacrylamide (PA) Slide

5:57

Culturing Mammalian Cells on MEArrays for Functional Analysis

9:05

Using MEArrays to Analyze How Type I Collagen and P-cadherin Affects Keratin Expression in Multipotent Mammary Epithelial Progenitor Cells

11:05

Conclusion

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