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Nanyang Technological University

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

14.5K Views

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12:30 min

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December 17th, 2012

DOI :

December 17th, 2012

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Isabel E. Moller¹^,², Filomena A. Pettolino³, Charlie Hart¹, Edwin R. Lampugnani¹, William G.T. Willats⁴, Antony Bacic¹^,²

¹Australian Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, ²Plant Cell Biology Research Centre, School of Botany, University of Melbourne, ³CSIRO Plant Industry, Black Mountain Laboratories, ⁴Department of Plant Biology and Biotechnology, University of Copenhagen

Summary

A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.

Explore More Videos

Glycan Profiling

Plant Cell Wall Polymers

Heterogeneous Glycans

Glycosyl Residues

Isomeric Configuration

Anomeric Configuration

Non sugar Residues

Cell And Tissue Types

Environmental Cues

Reverse Genetics Approaches

Chapters in this video

0:05

Title

1:40

Tissue Collection & Preparation

3:01

Extraction of Cell Wall Glycans

4:20

Printing Microarrays

5:45

Probing of Glycan Microarrays

6:36

Quantification

7:49

Results: CoMPP Profiling of N. alata

11:17

Conclusion

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