Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

15.3K Views

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10:47 min

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October 28th, 2011

DOI :

10.3791/3273-v

October 28th, 2011

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Xuejun H. Parsons¹^,², Yang D. Teng³^,⁴, James F. Parsons¹^,², Evan Y. Snyder¹^,²^,⁵, David B. Smotrich¹^,²^,⁶, Dennis A. Moore¹^,²

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery, Harvard Medical School, ⁴Division of SCI Research, VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

Summary

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Human Neuronal Progenitors

Neurons

Pluripotent Human Embryonic Stem Cells

Small Molecule Induction

Cell based Therapies

Central Nervous System CNS

Neural Stem Cells

Expansion Ability

Plasticity

Trophic And Neuroprotective Molecules

Pluripotent HESCs

Neurological Disorders

Differentiation Potential

Developmental Study

Clinical Translation

Multi lineage Inclination

Germ Layer

Chapters in this video

0:05

Title

1:27

Media Preparation and Plate Coating

2:33

Passaging and Seeding Undifferentiated Human ES Cells

4:31

Neural Induction in Adhesive Culture with Retinoic Acid

5:42

Differentiation in Suspension Culture and Maturation to Neuronal Phenotype after Attachment

8:08

Direct Neuronal Differentiation of Feeder- and Serum- Free Pluripotent Human ES Cells WIth Retinoic Acid Induction

10:11

Conclusion

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