Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

408.5K Views

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22:27 min

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May 6th, 2010

DOI :

10.3791/1869-v

May 6th, 2010

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Nynke L. van Berkum*¹, Erez Lieberman-Aiden*²^,³^,⁴^,⁵, Louise Williams*², Maxim Imakaev⁶, Andreas Gnirke², Leonid A. Mirny³^,⁶, Job Dekker¹, Eric S. Lander²^,⁷^,⁸

¹Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, ²Broad Institute of Harvard and Massachusetts Institute of Technology, ³Division of Health Sciences and Technology, Massachusetts Institute of Technology, ⁴Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University , ⁵Department of Applied Mathematics, Harvard University , ⁶Department of Physics, Massachusetts Institute of Technology, ⁷Department of Systems Biology, Harvard Medical School, ⁸Department of Biology, Massachusetts Institute of Technology

Summary

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Hi C

Three dimensional Architecture Of Genomes

Chromosomal Interactions

Chromosome Conformation Capture 3C

Genome wide Studies

Hi C Method

DNA protein Cross links

Biotinylated Residue

Ligation Products

Chapters in this video

0:00

Title

1:03

Introduction

2:07

Crosslinking, Digestion, Marking of DNA Ends, and Blunt-End Ligation

9:46

Shearing and Size Selection

12:03

Biotin Pull-Down and Paired End Sequencing

16:38

Representative Hi-C Results

21:32

Conclusion

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