Whole-Mount Immunostaining and Automatic Counting of Mouse Retinal Ganglion Cells

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05:52 min

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October 4th, 2024

DOI :

10.3791/66991-v

October 4th, 2024

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Jialiang Yang*¹, Jiaxin Guo*¹, Jing Hu*¹^,², Xiawei Wu*¹, Haotian Huang¹, Yu Wen¹, Runfang Chen¹, Congrui Liu¹, Houbin Zhang¹^,³

¹Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, ²School of Medicine, University of Electronic Science and Technology of China, ³Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

Podsumowanie

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Whole Mount Immunostaining

Automatic Counting

Mouse Retinal Ganglion Cells

Retinal Ganglion Cells RGCs

Vision Restoration

Therapeutic Interventions

Quantification Of RGC Numbers

Research Protocol

Rozdziały w tym wideo

0:00

Introduction

1:00

Isolation and Processing of Whole‐Mount Retinas for Immunostaining of Mouse Retinal Ganglion Cells

3:23

Immunostaining and Automated Counting of Mouse Retinal Ganglion Cells Using an AI‐Based Program

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