Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

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1.7K Views

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12:54 min

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May 19th, 2023

DOI :

10.3791/64874-v

May 19th, 2023

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Fatima Liaqat¹, Richard Thiga Kangethe¹, Rudolf Pichler¹, Bo Liu¹, Johann Huber², Viskam Wijewardana¹, Giovanni Cattoli¹, Luca Porfiri¹

¹Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, ²Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna

Podsumowanie

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Vaccine Immunogenicity

Bovine Monocyte derived Dendritic Cells

In Vitro Assay

Antigen presenting Cells

Intracellular Immune Mechanisms

Vaccine Efficacy

Lymphocyte Isolation Medium

Peripheral Blood Mononuclear Cells

Culture Medium

Flow Cytometry Analysis

CD14 MicroBead Buffer

FITC Staining Antibody

Rozdziały w tym wideo

0:04

Introduction

1:09

Production of Naive Bovine Monocyte-Derived Dendritic Cells (MoDCs)

6:01

Generation of Antigen-Pulsed MoDCs

7:20

MoDC Lymphocyte Co-Culture

8:20

Flow Cytometric Analysis

9:47

Results: In Vitro Generation of Cattle MoDCs for the Evaluation of Candidate Vaccine Antigens

12:10

Conclusion

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