JoVE Journal

Biology

Masz pełny dostęp do tej treści za pośrednictwem

Nanyang Technological University

Design and Implementation of a Rat Ex Vivo Lung Perfusion Model

Please note that all translations are automatically generated. Click here for the English version.

716 Views

•

04:38 min

•

May 26th, 2023

DOI :

10.3791/64740-v

May 26th, 2023

•

William J. Cleveland*¹, Josephine E. Hees*¹^,², Claudius Balzer¹^,², Hunter F. Douglas¹, Troy Stevens³^,⁴^,⁵, Matthias L. Riess¹^,⁶^,⁷

¹Anesthesiology, Vanderbilt University Medical Center, ²Anesthesiology, University of Greifswald, ³Department of Physiology and Cell Biology, University of South Alabama, ⁴Department of Medicine, University of South Alabama, ⁵Center for Lung Biology, University of South Alabama, ⁶Anesthesiology, Tennessee Valley Healthcare System Veterans Affairs Medical Center, ⁷Pharmacology, Vanderbilt University

Podsumowanie

Ex vivo lungs are useful for a variety of experiments to collect physiological data while excluding the confounding variables of in vivo experiments. Commercial setups are often expensive and limited in the types of data they can collect. We describe a method for building a fully modular setup, adaptable for various study designs.

Przeglądaj więcej filmów

Ex Vivo Lung Perfusion

Controlled Environment

Respiratory Anatomy

Tracheostomy Procedure

Surgical Protocol

Pulmonary Vasculature

Euthanasia Procedure

Diaphragm Incision

Heparin Injection

Rozdziały w tym wideo

0:00

Introduction

1:03

Lung Isolation Procedure

4:02

Results: Analyzing Lung Viability After Ischemia and Reperfusion

4:17

Conclusion

Powiązane Filmy

article

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

27.2K Views

article

A Small Animal Model of Ex Vivo Normothermic Liver Perfusion

13.3K Views

article

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

9.4K Views

article

A Rat Lung Transplantation Model of Warm Ischemia/Reperfusion Injury: Optimizations to Improve Outcomes

2.9K Views

article

Isolated Lung Perfusion System in the Rabbit Model

3.6K Views

article

Rat Model of Normothermic Ex-Situ Perfused Heterotopic Heart Transplantation

986 Views

article

Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation

621 Views

article

Exploring Alternative Perfusion Solutions Using Next-Generation Polymerized Hemoglobin-Based Oxygen Carriers in a Model of Rat Ex Vivo Lung Perfusion

469 Views

article

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death

357 Views

article

Perfuzja płuc ex vivo: technika badań płuc na modelu królika

1.2K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej