Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

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3.9K Views

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13:30 min

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February 18th, 2022

DOI :

10.3791/63583-v

February 18th, 2022

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Frederik Steiert¹^,², Tamara Heermann¹, Nikolas Hundt³, Petra Schwille¹

¹Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, ²Department of Physics, Technical University Munich, ³Department of Cellular Physiology, Biomedical Center (BMC), Ludwig-Maximilians-Universität München

Podsumowanie

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Mass sensitive Particle Tracking

Biomolecules

Lipid Membranes

Dynamics

Label free Observation

Oligomeric States

Membrane associated Systems

Ligand induced Receptor Complexes

Small Unilamellar Vesicles SUVs

Flow Chamber

Rozdziały w tym wideo

0:05

Introduction

0:43

Cleaning of Microscope Slides

1:48

Assembly of Flow Chambers

2:41

Supported Lipid Bilayer (SLB) Formation

3:31

Imaging

4:31

Data Analysis

10:25

Results: Native and Ratiometric View of Exemplary Surfaces

11:01

Results: Calibration of the Mass-to-Contrast Relation for MSPT Measurements

12:15

Results: Deciphering Oligomer States of Membrane-Associated Proteins

12:57

Conclusion

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