A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

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7.7K Views

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07:50 min

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March 30th, 2020

DOI :

10.3791/60806-v

March 30th, 2020

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Dinesh Jaishankar¹, Cormac Cosgrove¹, Ryan J. Deaton², I. Caroline Le Poole¹^,³

¹Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine, Northwestern University, ²Department of Pathology, University of Illinois at Chicago, ³Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University

Podsumowanie

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Multispectral Fluorescence Imaging

Frozen Tissue Sections

Biomarkers

Experimental Staining Method

Single Marker Staining

Control Unstained Slide

PBS Washes

DAPI Counterstaining

Spectral Library

Multispectral Imaging System

Machine Learning Software

Rozdziały w tym wideo

0:05

Introduction

0:39

Antibody and Fluorophore Selecting and Staining

1:52

Spectral Library Preparation

2:59

Multispectral Imaging

3:54

Spectral Unmixing

4:37

Analyzing Multispectral Images via Cell Segmentation and Phenotyping

5:52

Results: Representative Multispectral Imaging and Cell Quantification

7:07

Conclusion

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