Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

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14.1K Views

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07:41 min

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October 23rd, 2019

DOI :

10.3791/60424-v

October 23rd, 2019

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Baoyi Zhou¹, Marketa Kaucka¹, Andrei S. Chagin¹^,², Phillip T. Newton¹^,³

¹Department of Physiology and Pharmacology, Karolinska Institutet, ²Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), ³Department of Women's and Children's Health, Karolinska Institutet and Pediatric Endocrinology Unit, Karolinska University Hospital

Podsumowanie

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Clonal Genetic Tracing

Confetti Mouse

Fluorescent Proteins

Mineralized Tissues

Regenerative Medicine

Developmental Biology

Tissue Collection

Fluorescence Preservation

Cellular Populations

Imaging Protocol

EDTA Solution

Formaldehyde Fixation

Optimal Cutting Temperature OCT

Cryomold Embedding

Incubation Process

Rozdziały w tym wideo

0:04

Title

1:07

Tissue Fixation and Processing

2:50

Sectioning and Slide Preparation

4:09

Confocal Microscopy

5:42

Results: Confetti Images from a Col2CreERT: Confetti Mouse

6:47

Conclusion

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