JoVE Logo
Autorzy
Skontaktuj Się z Nami

Zaloguj się

JoVE Journal

Developmental Biology

Masz pełny dostęp do tej treści za pośrednictwem

Nanyang Technological University

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

11.2K Views

•

08:10 min

•

December 14th, 2015

DOI :

10.3791/53162-v

December 14th, 2015

•
1Department of Biomedical Science, The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine, Newcastle University, 3Department of Cardiovascular Science, The Bateson Centre, University of Sheffield, 4School of Biochemistry, University of Bristol, 5Biology Department, University of York

The manuscript here provides a simple set of methods for analysing the secretion and diffusion of fluorescently tagged ligands in Xenopus. This provides a context for testing the ability of other proteins to modify ligand distribution and allowing experiments that may give insight into mechanisms regulating morphogen gradients.

Przeglądaj więcej filmów

Keywords Xenopus Laevis
Confocal Analysis
Wnt
Sonic Hedgehog Shh
Morphogen Gradients
GFP tagged Ligands
MRNA Injection
Animal Caps
Lineage Tracing
Fluorescence Imaging
Quantitative Gradient Analysis

Rozdziały w tym wideo

0:05

Title

1:09

Microinjecting Xenopus Embryos

2:10

Excising Animal Cap Explants

4:17

Animal Explant Imaging

6:04

Results: Diffusion Analysis of Fluorescently Tagged Ligands in Xenopus Animal Cap Explants

7:38

Conclusion

Powiązane Filmy

article

11:13

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

7.9K Views

article

11:29

Technique to Target Microinjection to the Developing Xenopus Kidney

11.2K Views

article

07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

7.8K Views

article

10:05

Neural Explant Cultures from Xenopus laevis

12.5K Views

article

10:22

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

13.8K Views

article

05:36

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

13.8K Views

article

06:32

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

6.2K Views

article

00:09

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

7.9K Views

article

06:51

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

15.0K Views

article

Flow Cytometry and Confocal Imaging Analysis of Low Wnt Expression in Axin2-mTurquoise2 Reporter Thymocytes

1.4K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Skontaktuj Się z Nami

Poleć Bibliotece

BIULETYNY JoVE

Badania

ISSN 2578-2746

Edukacja

Autorzy

Bibliotekarze

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej