Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

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13.5K Views

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07:18 min

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July 26th, 2019

DOI :

10.3791/59687-v

July 26th, 2019

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Malhar Desai¹^,², Rong Di³, Huizhou Fan¹^,²

¹Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, ²Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University, ³Department of Plant Biology, School of Environmental and Biological, Rutgers University

Podsumowanie

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Biolayer Interferometry

BLI

Protein protein Interactions

Rozdziały w tym wideo

0:04

Title

0:59

Biosensor Hydration and Assay Set-Up

2:51

Loading of Ligand onto Biosensor and Washing Away Additional Ligand

3:43

Association of Analyte to Ligand and Dissociation of Analyte from Ligand

5:41

Results: A 28 Amino Acid Middle Region of GrgA Binds σ²⁸ In Vitro

6:45

Conclusion

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