Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

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9.3K Views

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09:30 min

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August 6th, 2018

DOI :

10.3791/57902-v

August 6th, 2018

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Alexandra J. Machen¹, Pierce T. O'Neil¹, Bradley L. Pentelute², Maria T. Villar¹, Antonio Artigues¹, Mark T. Fisher¹

¹Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, ²Department of Chemistry, Massachusetts Institute of Technology

Podsumowanie

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Keywords Protein Complexes

Biolayer Interferometry

PH induced Structural Rearrangements

Rozdziały w tym wideo

0:04

Title

1:28

Assembly of Prepore Anthrax Toxin Complex on a PDEA-modified Amine Reactive Biosensor Surface

4:37

Assembly of Pore Anthrax Toxin Complex on PDEA-modified Amine Reactive Biosensor Surface

5:26

Identification of Released Macromolecular Assemblies from BLI Biosensors by Negative Stain Electron Microscopy and Mass Spectrometry

6:50

Results: Analysis of Dynamic Protein Complexes by Biolayer Interferometry, Electron Microscopy, and Mass Spectrometry

8:29

Conclusion

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