Preparation of Exosomes for siRNA Delivery to Cancer Cells
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09:59 min
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December 5th, 2018
DOI :
December 5th, 2018
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0:04
Title
0:51
Bioreactor Flask Cell Culture
1:52
Conditioned Medium (CM) Harvesting from the Bioreactor Flask
2:41
Exosome Isolation onto a Sucrose Cushion
5:02
siRNA Encapsulation into Exosomes by Electroporation
6:11
Removal of Free siRNA Using Size Exclusion Chromatography (SEC)
7:17
Results: Effective Intracellular Delivery of siRNA Using siRNA-encapsulated Exosomes
9:11
Conclusion
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