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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

8.6K Views

06:38 min

February 7th, 2019

DOI :

10.3791/58763-v

February 7th, 2019


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Transcription Factor

Rozdziały w tym wideo

0:04

Title

1:00

Oligomer Annealing

1:45

Gel Preparation

3:35

Addition of the Competitor DNA Solution and Image Acquisition

4:41

Competitive Titration Assay and the Fitting Procedure

5:17

Results: Determining TF-DNA Binding Affinities Using Fluorescence Microscopy

6:07

Conclusion

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