Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
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09:17 min
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January 11th, 2017
DOI :
January 11th, 2017
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Rozdziały w tym wideo
0:05
Title
0:53
Setting up the 96-well Dot Blot Apparatus
2:02
Reaction Initiation and Quenching
2:57
Spotting of Quenched Reactions on Nitrocellulose
4:51
Nitrocellulose Processing
5:48
Preparing Nitrocellulose Membrane for Scintillation Counting
7:31
Results: Quantification of the Autophosphorylation of V. parahaemolyticus H-NOX Associated Histidine Kinase (Vp1876)
8:35
Conclusion
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