JoVE Journal

Environment

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Nanyang Technological University

An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients

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15.7K Views

•

07:45 min

•

October 22nd, 2018

DOI :

10.3791/58674-v

October 22nd, 2018

•

Jennifer E. Schmidt¹, Carolyn Lowry², Amelie C.M. Gaudin¹

¹Department of Plant Sciences, University of California at Davis, ²Department of Natural Resources and the Environment, University of New Hampshire

Podsumowanie

Visualizing and measuring root growth in situ is extremely challenging. We present a customizable rhizobox method to track root development and proliferation over time in response to nutrient enrichment. This method is used to analyze maize genotypic differences in root plasticity in response to an organic nitrogen source.

Przeglądaj więcej filmów

Rhizobox Protocol

Localized Nutrients

Root And Rhizosphere Science

Non invasive Measurements

Polyester Padding

Treatment Patches

Control Patches

Nitrogen Source

Experimental Setup

Rozdziały w tym wideo

0:04

Title

0:27

Assembly of the Rhizoboxes

2:08

Substrate Preparation for Treatment and Control Patches

2:54

Loading the Rhizobox with Substrate and Establishing Treatment and Control Patches

5:02

Seed Transplantation and Plant Growth

6:19

Results: Roots Proliferate in Treated Patches

7:26

Conclusion

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