Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

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15.6K Views

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07:11 min

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January 7th, 2019

DOI :

10.3791/58601-v

January 7th, 2019

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Louisa Jutta Dietz¹, Anuroop Venkateswaran Venkatasubramani¹, Annika Müller-Eigner², Martin Hrabe de Angelis³^,⁴^,⁵, Axel Imhof¹, Lore Becker³, Shahaf Peleg²^,⁶^,⁷

¹Munich Center of Integrated Protein Science and Biomedical Center, Ludwig-Maximilians University of Munich, ²Laboratory for Metabolism and Epigenetics in Aging, Leibniz Institute for Farm Animal Biology (FBN), ³German Mouse Clinic, Helmholtz Zentrum Munich, German Research Center for Environment and Health (GmbH), ⁴German Center for Diabetes Research (DZD), ⁵Chair of Experimental Genetics, School of Life Science Weihenstephan, Technische Universität München, ⁶Laboratory for Metabolism and Epigenetics in Brain Aging, Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, ⁷Molecular Biology Division, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians University of Munich

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Keywords Oxygen Consumption Rate

Metabolism

Aging

Mitochondrial Isolation

Calibration

Experimental Drugs

Fly Head Segments

Rozdziały w tym wideo

0:04

Title

0:33

Instrument Preparation

1:24

Cartridge Preparation

2:21

Plate Preparation

4:37

Analysis of OCR Measurements

5:12

Results: Oxygen Levels of Midlife Heads Lower than Young Heads

6:42

Conclusion

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