Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

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11.2K Views

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08:29 min

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November 30th, 2018

DOI :

10.3791/58387-v

November 30th, 2018

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Heidi A. Rossow¹, Gabriela Acetoze², John Champagne¹, Jon J. Ramsey³

¹Veterinary Medicine Teaching and Research Center, University of California Davis, ²Archer Daniels Midland, ³Department of Molecular Biosciences, University of California Davis

Podsumowanie

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Keywords Mitochondrial Oxygen Consumption

Proton Leak Kinetics

Mitochondrial Respiration

Dairy Cattle

Energetics

Mitochondrial Isolation

Liver Sample

Homogenization

Centrifugation

Protein Concentration

Oxygen Consumption Assay

Respiration Chamber

Oxygraph System

Rozdziały w tym wideo

0:04

Title

0:54

Isolating Mitochondria from Dairy Cow Liver

2:46

Measuring Mitochondrial Oxygen Consumption

4:33

Measuring Mitochondrial Membrane Potential (MMP) and Proton Motive Force (PMF)

6:42

Results: Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics

7:45

Conclusion

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