Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

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09:12 min

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January 18th, 2019

DOI :

10.3791/58542-v

January 18th, 2019

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Eva Königshausen¹, Sebastian A. Potthoff¹, Raphael Haase¹, Catherine Meyer-Schwesinger², Ernest Kaufmann¹, L. Christian Rump¹, Johannes Stegbauer¹, Lorenz Sellin¹, Ivo Quack¹, Magdalena Woznowski¹

¹Department of Nephrology, Medical Faculty, Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

Podsumowanie

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Keywords Glomeruli Isolation

In Vivo Labeling

Glomerular Cell Surface Proteins

Proteinuric Kidney Disease

Cell Surface Protein Trafficking

Rozdziały w tym wideo

0:04

Title

0:47

Surgical Procedure

3:00

In Vivo Biotinylation and Glomeruli Isolation

5:29

Glomeruli Washing and Quantification

6:25

Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins

8:31

Conclusion

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