Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

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06:45 min

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January 11th, 2019

DOI :

10.3791/58461-v

January 11th, 2019

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Hanan B. Elajaili¹, Laura Hernandez-Lagunas¹, Kalina Ranguelova², Sergey Dikalov³, Eva Nozik-Grayck¹

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center

Podsumowanie

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Keywords Electron Paramagnetic Resonance

EPR Spectroscopy

Redox Biology

Reactive Oxygen Species

Spin Probes

Superoxide Detection

264 7 Cells

Krebs Henseleit Buffer

Rozdziały w tym wideo

0:04

Title

1:05

RAW 264.7 Cell Phorbol 12-Myristate 13-Acetate (PMA)-Treatment

2:27

Superoxide Detection in Buffer or Cell Samples

3:58

EPR Measurements on Flash Frozen Lung Tissue

4:57

Results: Representative Superoxide Detection

6:21

Conclusion

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