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Medicine

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Nanyang Technological University

A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence

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9.5K Views

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09:21 min

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October 11th, 2018

DOI :

10.3791/57523-v

October 11th, 2018

•

Eric Parent¹, Patrick Burns², André Desrochers¹, Martine Boulianne¹

¹Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, ²University of Bristol

Podsumowanie

Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.

Przeglądaj więcej filmów

Ligated Intestinal Loop Model

Anesthetized Chickens

Clostridium Perfringens

Chicken Necrotic Enteritis

Intestinal Pathogens

Surgical Procedure

Skin Preparation

Abdominal Incision

Intestine Exteriorization

Rozdziały w tym wideo

0:04

Title

0:56

Preparations

1:42

Extraction of the Small Intestines from the Abdominal Cavity

3:11

Fabricating Intestinal Loops and Injecting them with Pathogens

4:58

Completing the Procedure and Collecting Results

6:44

Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion

8:02

Conclusion

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