JoVE Journal

Immunology and Infection

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Nanyang Technological University

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

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3.2K Views

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09:48 min

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July 27th, 2022

DOI :

10.3791/64215-v

July 27th, 2022

•

Heather Liebe¹, Camille Schlegel², Xue Cai², Alena Golubkova¹, Tyler Leiva¹, William L. Berry³, Catherine J. Hunter¹

¹Division of Pediatric Surgery, Oklahoma Children's Hospital, ²Oklahoma University Health Sciences Center, ³Department of Surgery, University of Oklahoma Health Sciences Center

Podsumowanie

The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.

Przeglądaj więcej filmów

Intestinal Permeability

Apical Out Enteroid Model

Necrotizing Enterocolitis

Paracellular Pathways

Cell Recovery Solution

Tissue Culture Plate

Experimental Procedures

Post Doctoral Research Scholars

Adult Enteroids Incubation

Centrifuge Method

Rozdziały w tym wideo

0:04

Introduction

0:59

Generation of Apical‐Out (AO) Enteroids

3:08

Induction of Experimental Necrotizing Enterocolitis (NEC)

5:06

Measurement of Paracellular Permeability Utilizing Lucifer Yellow (LY)

7:49

Results: Determining the Intestinal Permeability Using Lucifer Yellow in an Apical‐Out Enteroid Model

9:07

Conclusion

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