Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

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11.1K Views

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10:20 min

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August 10th, 2018

DOI :

10.3791/57466-v

August 10th, 2018

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Zhaofeng Jia¹^,²^,³, Yujie Liang⁴^,⁵, Xingfu Li²^,³, Xiao Xu¹^,²^,³, Jianyi Xiong²^,³, Daping Wang¹^,²^,³, Li Duan²^,³

¹Postgraduate institution, Guangzhou Medical University, ²Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, ³Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ⁴Department of Chemistry, Chinese University of Hong Kong, ⁵Shenzhen Kangning Hospital, Shenzhen Mental Health Center

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Keywords Magnetic activated Cell Sorting

Mesenchymal Stem Cells

Cell Suspension

Rozdziały w tym wideo

0:04

Title

1:07

Isolate and Culture the rbSF-MSCs

3:21

CD90-positive Magnetic Activated Cell Sorting (MACS) of the rbSF-MSCs and Primary Culture

5:01

Magnetic Separation and Culture of te CD90⁺ rbSF-MSCs

6:44

Results: Obtain Purified Rabbit SF-MSCs by CD90-Based Magnetic Activated Cell Sorting

8:39

Conclusion

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