JoVE Journal

Neuroscience

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Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

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9.5K Views

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08:23 min

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May 12th, 2018

DOI :

10.3791/57391-v

May 12th, 2018

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Emily D. Geyer*¹, Prithvi A. Shetty*¹, Christopher J. Suozzi*¹, David Z. Allen*¹^,², Pamela P. Benavidez*¹^,², Joseph Liu*¹^,³, Charles N. Hollis¹, Greg A. Gerhardt⁴, Jorge E. Quintero⁴, Jason J. Burmeister⁴, Emmett E. Whitaker¹^,³

¹Department of Anesthesiology, Ohio State University College of Medicine, ²Medical Student Research Program, Ohio State University College of Medicine, ³Department of Anesthesiology and Pain Medicine, Nationwide Children's Hospital, ⁴Department of Neuroscience, University of Kentucky Medical Center

Podsumowanie

This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.

Przeglądaj więcej filmów

Microelectrode Array

Neurotransmitters

Anesthesia induced Neurotoxicity

In Vivo Glutamate Activity

Pediatric Brain Trauma

Sevoflurane Anesthesia

Stereotaxic Frame

Vital Signs Monitoring

Rozdziały w tym wideo

0:04

Title

1:19

Set Up for Surgery

3:38

Microelectrode Array Placement

6:19

Results: In Vivo Glutamate Measurements from the Piglet Hippocampus

7:56

Conclusion

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