Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

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8.8K Views

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10:12 min

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May 15th, 2018

DOI :

10.3791/56394-v

May 15th, 2018

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Shukun Chen*¹, Amin El-Heliebi*¹, Julia Schmid¹, Karl Kashofer², Zbigniew T. Czyż³, Bernhard Michael Polzer³, Klaus Pantel⁴, Thomas Kroneis¹^,⁵, Peter Sedlmayr¹

¹Institute of Cell Biology, Histology and Embryology, Medical University of Graz, ²Institute of Pathology, Medical University of Graz, ³Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, ⁴Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, ⁵Sahlgrenska Cancer Center, University of Gothenburg

Podsumowanie

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Keywords Single Cell

Cell Suspension

Liquid Biopsy

Cellular Heterogeneity

Circulating Tumor Cells

Cell Characterization

Cell Isolation

Cell Harvesting

Whole Genome Amplification

Fluorescence Microscopy

Cell Counting

Cell Resuspension

Peripheral Blood

Rozdziały w tym wideo

0:05

Title

1:28

Preparation of Target Cells

2:41

Charging the Wire

3:27

Counting Cells on the Wire

4:03

Detachment and Recovery of Target Cells

5:25

Single-cell Sampling Using Micromanipulation

6:37

Single-cell Sampling Using Laser Microdissection

7:47

Results: Use of Functionalized Wire for Ex Vivo Characterization and Genomic Analysis at Single Cell Level

8:59

Conclusion

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