Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

8.8K Views

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10:12 min

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May 15th, 2018

DOI :

10.3791/56394-v

May 15th, 2018

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Shukun Chen*¹, Amin El-Heliebi*¹, Julia Schmid¹, Karl Kashofer², Zbigniew T. Czyż³, Bernhard Michael Polzer³, Klaus Pantel⁴, Thomas Kroneis¹^,⁵, Peter Sedlmayr¹

¹Institute of Cell Biology, Histology and Embryology, Medical University of Graz, ²Institute of Pathology, Medical University of Graz, ³Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, ⁴Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, ⁵Sahlgrenska Cancer Center, University of Gothenburg

Summary

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Keywords Single Cell

Cell Suspension

Liquid Biopsy

Cellular Heterogeneity

Circulating Tumor Cells

Cell Characterization

Cell Isolation

Cell Harvesting

Whole Genome Amplification

Fluorescence Microscopy

Cell Counting

Cell Resuspension

Peripheral Blood

Chapters in this video

0:05

Title

1:28

Preparation of Target Cells

2:41

Charging the Wire

3:27

Counting Cells on the Wire

4:03

Detachment and Recovery of Target Cells

5:25

Single-cell Sampling Using Micromanipulation

6:37

Single-cell Sampling Using Laser Microdissection

7:47

Results: Use of Functionalized Wire for Ex Vivo Characterization and Genomic Analysis at Single Cell Level

8:59

Conclusion

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