Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Please note that all translations are automatically generated. Click here for the English version.
4.6K Views
•
12:41 min
•
December 23rd, 2022
DOI :
December 23rd, 2022
•Maxim Le Compte1, Edgar Cardenas De La Hoz2, Sofía Peeters1, Evelien Smits1, Filip Lardon1, Geert Roeyen1,3, Steve Vanlanduit2, Hans Prenen1,4, Marc Peeters1,4, Abraham Lin1,5, Christophe Deben1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Introduction
0:53
Day 0: Preparation of the 2- or 3-Day-Old Organoids
2:55
Days 2 - 3: Harvesting the 2- or 3-Day-Old Organoids
4:16
Seeding the 2- or 3-Day-Old Organoids Using a Pipetting Robot
5:27
Day 4: Drug Treatment and Reagent Dispensing with Digital Drug Dispenser
7:49
Image Acquisition with the Live-Cell Imager and Analysis of Data
10:12
Results: The Single-Organoid Dose-Response Analysis
12:03
Conclusion
Powiązane Filmy
11:20
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
10.8K Views
10:04
Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
11.9K Views
05:54
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
8.5K Views
09:36
Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
10.1K Views
05:58
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
10.8K Views
08:32
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates
9.0K Views
09:17
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
7.6K Views
09:43
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
6.4K Views
07:20
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
9.8K Views
09:50
Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
7.9K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone