Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

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9.9K Views

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06:24 min

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December 15th, 2017

DOI :

10.3791/56234-v

December 15th, 2017

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Johannes Hemmerich*¹^,², Lars Freier*¹^,², Wolfgang Wiechert¹^,²^,³, Eric von Lieres¹^,², Marco Oldiges¹^,²^,⁴

¹IBG-1: Biotechnology, Forschungszentrum Jülich, ²Research Center Jülich, Bioeconomy Science Center (BioSC), ³Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, ⁴Institute for Biotechnology, RWTH Aachen University

Podsumowanie

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Keywords Heterologous Protein Production

Automated Microbioreactor Technology

Kringing Based Experimental Design

Microbial Cultivation Media Optimization

Bioprocess Development

Cultivation Microtiter Plates

Biolector Device

Stock Solutions Disposal

Labware Cleaning

Liquid Handler Decontamination

Rozdziały w tym wideo

0:05

Title

1:05

Seed Culture, Automated Media Preparation, and Start of Main Culture

2:53

Product Quantification and Preprocessing of Raw Data for Analysis

4:22

Results: Iterative Workflow with Final Statistical Evaluation of Results

5:31

Conclusion

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