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JoVE Journal

Bioengineering

This content is Open Access.

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

34.4K Views

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10:47 min

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June 11th, 2013

DOI :

10.3791/50356-v

June 11th, 2013

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1Application Development, Akonni Biosystems, Inc., 2Manufacturing, Akonni Biosystems, Inc., 3Engineering, Akonni Biosystems, Inc., 4Research & Development, Akonni Biosystems, Inc.

TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.

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TruTip Technology
Nucleic Acid Extraction
Automated Sample Processing
Clinical Samples
Liquid Handling Robots
RNA Isolation
Genomic DNA Isolation
Fetal DNA Extraction
Eppendorf EpMotion 5070
Hamilton STAR
STARplus
High throughput
Porous Monolithic Binding Matrix
Cell Lysis
Nucleic Acid Binding
Washing
Elution

Rozdziały w tym wideo

0:05

Title

2:15

The Hamilton STAR liquid-handling Robot

2:51

Setting Up a 96-well DNA Extraction from Whole Blood

5:02

Sample Lysis and Reagent Distribution

7:31

gDNA Extraction

9:12

Nucleic Acid Extraction, Speed, Fidelity and Purity

10:18

Conclusion

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