Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

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7.2K Views

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11:59 min

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September 6th, 2017

DOI :

10.3791/56002-v

September 6th, 2017

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Roman Crazzolara¹, Gabriele Kropshofer¹, Michael Steurer², Sieghart Sopper²^,³, Wolfgang Schwinger⁴

¹Department of Pediatrics, Medical University Innsbruck, ²Department of Internal Medicine V (Hematology & Oncology), Medical University Innsbruck, ³Tyrolean Cancer Research Institute, ⁴Department of Pediatrics, Medical University Graz

Podsumowanie

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Keywords Hematopoietic Stem Cell Transplantation

Hemoglobinopathies

Chimerism

Erythroid Progenitor Cells

Immunomodulatory Strategies

Rozdziały w tym wideo

0:05

Title

1:22

Isolation of Hematopoietic Bone Marrow cells by Multi-parameter Fluorescence-Actviated Cell Sorting (FACS)

6:33

Clonogenic Assay

7:54

Analysis of Chimerism by PCR

9:16

Results: Quantifying Relative Proportions of Donor-versus-recipient Chimerism in the Erythroid Progenitors

10:54

Conclusion

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