Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

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14.2K Views

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08:52 min

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January 10th, 2018

DOI :

10.3791/55848-v

January 10th, 2018

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Kritika S. Katiyar*¹^,²^,³, Carla C. Winter*¹^,²^,⁴, Wisberty J. Gordián-Vélez¹^,²^,⁴, John C. O'Donnell¹^,², Yeri J. Song¹^,⁵, Nicole S. Hernandez¹^,⁵, Laura A. Struzyna¹^,²^,⁴, D. Kacy Cullen¹^,²^,⁵

¹Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, ²Center for Neurotrauma, Neurodegeneration & Restoration, Michael J. Crescenz Veterans Affairs Medical Center, ³School of Biomedical Engineering, Drexel University, ⁴Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, ⁵Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:03

Agarose Hydrogel Micro-Column Fabrication

3:51

ECM Core Fabrication

5:26

Seeding Micro-columns with Astrocytes

6:32

Results: Formation of Astrocyte Bundles within Hydrogel Micro-columns over Time

7:54

Conclusion

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