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Chemistry

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Nanyang Technological University

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

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9.3K Views

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08:41 min

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July 14th, 2017

DOI :

10.3791/55778-v

July 14th, 2017

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Xiaoguang Sunny Li¹, Shu Li¹, Melissa Ahrens², Gottfried Kellermann¹^,²

¹Pharmasan Labs, Inc., ²NeuroScience, Inc.

Podsumowanie

A selective and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method coupled with an efficient solid phase extraction on a mixed-mode cation-exchange (MCX) 96-well microplate was developed for the measurement of free 3-nitrotyrosine (3-NT) in human urine with high throughput, which is suitable for clinical applications.

Przeglądaj więcej filmów

3 nitrotyrosine

Solid Phase Extraction

Oxidative Stress

Clinical Application

Rozdziały w tym wideo

0:05

Title

1:41

Solid Phase Extraction Procedure

7:47

Conclusion

6:17

Results: LC-MS/MS Determination of 3-Nitrotyrosine in Human Urine

5:00

LC-MS/MS Analysis

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